RING finger and WD repeat domain 3 regulates proliferation and metastasis through the Wnt/β-catenin signalling pathways in hepatocellular carcinoma

Figure 8 shRFWD3i influences the progress of hepatocellular carcinoma through the Wnt/β-catenin signalling pathway. A: Heatmap analysis showing the gene expression changes after RING finger and WD repeat domain 3 (RFWD3) silencing (454 genes, above 2-fold); B: Disease and functional analysis to classify genes enriched after RFWD3 silencing; C: Classical pathway analysis indicates genes enriched after RFWD3 silencing; D: The microarray analysis reveals alteration in the Wnt/β-catenin signalling pathway following RFWD3 silencing; E: Knowledge-based interaction network of Wnt/β-catenin signalling targets (> 2-fold change, P < 0.05). The intensity of the node color indicates the degree of upregulation (red) or downregulation (green) following RFWD3 silencing in hepatocellular carcinoma cells; F-I: The expression of CREBBP, Wnt5A, JUN, Wnt7B and LRP6 were decreased, and the expression of KREMEN-1 was increased in the shRNAi group using western blot analysis, in rescue experiment, the results are reversed.