RING finger and WD repeat domain 3 regulates proliferation and metastasis through the Wnt/β-catenin signalling pathways in hepatocellular carcinoma

Figure 3 RING finger and WD repeat domain 3 knockdown inhibits cell growth and proliferation and induces cell cycle arrest and apoptosis. A: Cell growth and proliferation of BEL-7404, Hepatocellular carcinoma (HCC)-LM3 shRNAi, and shCtrl cells continuously monitored using a Celigo instrument for five days; B: MTT assay demonstrating that RING finger and WD repeat domain 3 siRNA significantly reduced the proliferation of HCC cells; C and D: The cell cycle progression evaluated by flow cytometry (FCM) with propidium iodide (PI) and RNase A (^bP < 0.01); E and F: FCM to assess the apoptosis in shRNAi and shCtrl groups of cells using Annexin V-APC/PI Apoptosis Detection Kit (^bP < 0.01).