Involvement of Met receptor pathway in aggressive behavior of colorectal cancer cells induced by parathyroid hormone-related peptide

Figure 8 Parathyroid hormone-related peptide increases the protein expression of Met and parathyroid hormone receptor type 1 in tumor xenografts. Met and parathyroid hormone-related peptide receptor type 1 (PTHR1) protein levels were evaluated by the immunohistochemistry technique in the HCT116 cell tumor-bearing nude mouse model. Tumor sections were stained with an anti-Met antibody or anti-PTHR1 antibody. Images (400 ×) are from the tumor treated with saline solution (left) or with PTHrP (right). The immunostaining was quantified with the Fiji image processing package of the Image J-NIH program. Scale bar: 50 μM. Met: Receptor tyrosine kinase Met; PTHR1: Parathyroid hormone receptor type 1; PTHrP: Parathyroid hormone-related peptide. ^aP < 0.05; ^bP < 0.01.