Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

Figure 2 The distribution and ultrastructural characteristics in porcine liver sinusoidal endothelial cells of the control liver. A: The representative image of the distribution of liver sinusoidal endothelial cells (LSEC) in the porcine liver indicated by the immunohistochemical staining with antibody to ERG. Bar = 10 μm; B: Typical LSEC were identified in the ultrathin sections (90 nm) of the Epon 812-embedded control liver tissue. Bars = 1 μm; C and D: LSEC was observed by scanning electron microscopy in osmium-macerated control porcine liver. s: sinusoid. h: Hepatocyte. The space of Disse was indicated by an asterisk. The partial area indicated in C was further observed in high magnification D. Bars = 1 μm. Mitochondria is represented in green and the nucleus is represented in blue. Arrows indicate the rough endoplasmic reticulum (rER). TEM: Transmission electron microscopy; SEM: Scanning electron microscopy.