Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway

Figure 6 Effects of intraperitoneal pancreatitis-associated ascitic fluid injection with or without anti-high mobility group box protein 1 neutralizing antibody on intestinal inflammation and cell apoptosis in cerulein-treated rats. A: Intestinal tumor necrosis factor-α; B: Intestinal interleukin-6; C: Statistical results of the number of apoptotic intestinal mucosal cells in each group; D: Representative images (400 × magnification) of terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) assay; E: Immunoblotting of Bax, Bcl-2, pro-caspase-3 and cleaved-caspase-3 protein expression from intestinal samples; F-H: Densitometry analysis of the immunoblotting data of apoptosis-related proteins in intestinal tissue. Data are expressed as means ± SD, n = 6 enzyme-linked immunosorbent assay and TUNEL assay results per group; means ± SD, n = 3 immunoblotting results per group. ^aP < 0.05 vs control group; ^bP < 0.05 vs pancreatitis-associated ascitic fluid group. TNF-α: Tumor necrosis factor-α; CN: Control; PI: Pancreatitis-associated ascitic fluid (PAAF) injection; PIH: PAAF and 200 μg anti-high mobility group box protein 1 neutralizing antibody; PIC: PAAF + control lgY; IL-6: Interleukin-6; TUNEL: Terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; DAPI: 4',6-diamidino-2-phenylindole.