Metabolomics of Fuzi-Gancao in CCl4 induced acute liver injury and its regulatory effect on bile acid profile in rats

Table 4 Recovery of the developed ultra performance liquid chromatography-tandem mass spectrometry method (n = 6)

Ingredient	Baseline (ng/mL)	Spiked (ng/mL)	Recovery %
L-Leu	1000	250	92.53 ± 7.13
		500	93.42 ± 11.52
		750	87.53 ± 9.59
5-HT	8	2	85.37 ± 9.79
		4	90.52 ± 13.47
		6	93.05 ± 10.16
N-phe	2500	625	94.54 ± 12.15
		1250	89.46 ± 6.54
		1875	86.72 ± 8.19
L-Try	6000	1500	93.04 ± 13.23
		3000	85.42 ± 7.12
		4500	91.16 ± 12.25
L-Kyn	50	12.5	90.48 ± 6.18
		25	85.30 ± 10.42
		37.5	86.20 ± 6.13
5-HTP	2	0.5	91.49 ± 5.36
		1	94.64 ± 7.46
		1.5	93.58 ± 5.15
CA	400	100	91.83 ± 12.12
		200	93.29 ± 8.62
		300	90.22 ± 7.28
GSH	1000	250	88.17 ± 6.33
		500	91.16 ± 6.15
		750	90.85 ± 9.81
GSSG	2000	500	91.15 ± 10.08
		1000	93.92 ± 12.15
		1500	89.27 ± 10.92

L-Leu: L-leucine; 5-HT: 5-Hydroxytryptamine; N-phe: N-phenylacetylglycine; L-Try: L-tryptophan; L-Kyn: L-kynurenine; 5-HTP: 5-hydroxytryptophan; CA: Cholic acid; GSH: Glutathione; GSSG: Glutathione oxidized.