Hepatitis B virus persistent infection-related single nucleotide polymorphisms in HLA regions are associated with viral load in hepatoma families

Table 3 Multivariate generalized estimating equation and area under the curve for hepatitis B surface antigen status in the hepatocellular carcinoma family cohort

	Nongenetic model		Mixed model
Variable	OR (95%CI)	P value	OR (95%CI)	P value
Sex, male	1.458 (1.048-2.027)	0.0250	1.514 (1.075-2.133)1/1.487 (1.063-2.081)2	0.01771/0.02052
Index sex, male	0.915 (0.532-1.573)	0.7475	0.853 (0.496-1.466)1/0.838 (0.488-1.442)2	0.56481/0.52382
Age in yr	1.001 (0.984-1.018)	0.9392	1.000 (0.982-1.017)1/0.998 (0.981-1.016)2	0.96081/0.85822
Relation to index
Parent generation	0.523 (0.176-1.555)	0.2434	0.560 (0.182-1.719)1/0.603 (0.198-1.833)2	0.31081/0.37262
Index generation	3.385 (1.836-6.239)	< 0.0001	3.344 (1.766-6.331)1/3.493 (1.860-6.559)2	0.00021/0.00012
Index’s HBsAg+	5.077 (3.103-8.308)	< 0.0001	4.919 (2.980-8.119)1/4.756 (2.912-7.766)2	< 0.00011/< 0.00012
Mother’s status
HBsAg+	2.597 (1.332-5.064)	0.0051	2.459 (1.270-4.760)1/2.517 (1.284-4.933)2	0.00761/0.00722
Unknown	1.395 (0.788-2.469)	0.2538	1.412 (0.790-2.522)1/1.413 (0.795-2.511)2	0.24441/0.23872
AUC (95%CI)	0.786 (0.752-0.820)	< 0.0001
	Genetic model
rs477515	1.303 (0.969-1.753)	0.0802	1.121 (0.770-1.631)	0.5507
rs9276370	2.741 (0.766-9.812)	0.1211	3.040 (0.623-14.839)	0.1693
rs7756516	0.592 (0.171-2.042)	0.4064	0.516 (0.104-2.554)	0.4177
rs9277535	1.575 (1.244-1.995)	0.0002	1.535 (1.157-2.035)	0.0029
AUC (95%CI)	0.632 (0.593-0.671)	< 0.0001	0.798 (0.765-0.831)	< 0.0001
WGRS	1.322 (1.162-1.505)	< 0.0001	1.269 (1.099-1.465)	0.0012
AUC (95%CI)	0.620 (0.580-0.660)	< 0.0001	0.795 (0.762-0.829)	< 0.0001
IDI (95%CI)			0.017 (0.009-0.026)	< 0.0001
NRI (95%CI)			0.330 (0.192-0.467)	< 0.0001

¹Each single nucleotide polymorphism was included in the mixed model.

²The weighted genetic risk score was added in the mixed model. AUC: Area under the receiver operating characteristic curve; CI: Confidence interval; OR: Odds ratio; GEE: Generalized estimating equation; IDI: Integrated discrimination improvement; NRI: Net reclassification improvement; WGRS: Weighted genetic risk score.