Recombinant angiopoietin-like protein 4 attenuates intestinal barrier structure and function injury after ischemia/reperfusion

Figure 7 Angiopoietin-like protein 4 deficiency aggravates Caco-2 cells injury challenge by hypoxia/reoxygenation. A and B: Reactive oxygen species (ROS) levels (scale bar = 12.5 μm) (A) determined by immunofluorescence and tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 levels (B) determined by enzyme-linked immunosorbent assay; C: Tight junction zonula occludens-1 (ZO-1) and claudin-2 (CLDN-2), autophagy marker microtubule-associated protein light chain 3 (LC3) II and apoptosis marker caspase-3 were determined using western blot assays (mean ± SD, n = 3). ^aP < 0.05, ^bP < 0.01 vs vehicle; ^cP < 0.05, ^dP < 0.01 vs vehicle (n = 3 per group). ANGPTL4: Angiopoietin-like protein 4; H/R: Hypoxia/reoxygenation; si: Small interfering RNA.