Insight into molecular mechanisms underlying hepatic dysfunction in severe COVID-19 patients using systems biology

Table 2 List of primer designs used in the qRT-PCR validation of some differentially expressed genes (DNAJB1, HSP90AB1, and CYP39A1) and housekeeping gene (18SrRNA)

Gene symbol	Forward primer sequence	Reverse primer sequence	Amplicon size (bp)
18SrRNA	TGACTCAACACGGGAAACC	TCGCTCCACCAACTAAGAAC	114
DNAJB1	GTTTTAAAGGACAAGCCCCACA	TCCAGAGTGGGGACGTTCA	119
HSP90AB1	CTCTGTCAGAGTATGTTTCTCGC	GTTTCCGCACTCGCTCCACAAA	114
CYP39A1	GTTCCAGTGTCCTGCAAGGT	TGGGTAATGGGTCCAGAAGAC	101

bp: base pair; 18SrRNA: 18S ribosomal RNA; DNAJB1: DnaJ heat shock protein family (Hsp40) member B1; HSP90AB1: Heat shock protein 90 alpha family class B member 1; CYP39A1: Cytochrome P450 family 39 subfamily A member 1.