Lipotoxic hepatocyte-derived exosomal miR-1297 promotes hepatic stellate cell activation through the PTEN signaling pathway in metabolic-associated fatty liver disease

Figure 3 Quantitative real-time PCR analysis verification for differentially expressed microRNAs in exosomes derived from primary hepatocytes and patient serum samples of metabolic-associated fatty liver disease. A: Quantitative real-time PCR was performed on exosomes derived from vehicle control treated primary hepatocyte and palmitic acid treated primary hepatocyte. The fold change ratio of microRNA (miRNA) in the Exo-PA group was compared to the result of differentially expressed miRNAs in the miRNA sequencing (miRNA seq). Detailed data of individual miRNA expression could be seen Supplementary Table 2; B: Quantitative real-time (q)PCR was performed between mild and severe fatty liver human serum exosome samples. The fold change ratio of miRNA in severe fatty liver were compared to the result of the differentially expressed miRNAs of the miRNA sequencing. Detailed data of individual miRNA expression could be seen Supplementary Table 2. MAFLD: Metabolic-associated fatty liver disease.