MiR-19a-3p regulates the Forkhead box F2-mediated Wnt/β-catenin signaling pathway and affects the biological functions of colorectal cancer cells

Figure 3 Effects of Forkhead box F2 on proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal cancer cells. A: Forkhead box F2 (FOXF2) had low expression in colorectal cancer cells; B: Compared with cells transfected with MiR negative control (miR-NC), cells transfected with sh-FOXF2 showed significantly upregulated expression of FOXF2, and cells transfected with si-FOXF2 showed significantly down-regulated expression of FOXF2; C: Compared with cells transfected with miR-NC, cells transfected with si-FOXF2 showed significantly stronger cell proliferation ability, while cells transfected with sh-FOXF2 showed significantly weaker cell proliferation ability; D: Compared with cells transfected with miR-NC, cells transfected with si-FOXF2 showed significantly stronger cell invasion ability, while cells transfected with sh-FOXF2 showed significantly weaker cell invasion ability; E: Compared with cells transfected with miR-NC, cells transfected with si-FOXF2 showed significantly stronger cell invasion ability, while cells transfected with sh-FOXF2 showed significantly weaker cell invasion ability; F: Compared with cells transfected with miR-NC, cells transfected with si-FOXF2 showed significantly up-regulated expression of p-GSK-3β and β-catenin, and significantly down-regulated expression of GSK-3β and p-β-catenin, and cells transfected with Sh-FOXF2 showed significantly downregulated expression of p-GSK-3β and β-catenin, and significantly upregulated expression of GSK-3β and p-β-catenin; G: Western Blot results; H: Compared with cells transfected with miR-NC, cells transfected with si-FOXF2 showed significantly up-regulated expression of N-cadherin and vimentin, and significantly down-regulated expression of E-cadherin, and cells transfected with sh-FOXF2 showed significantly down-regulated expression of N-cadherin and vimentin and significantly up-regulated expression of E-cadherin; I: Western blot results. Note: a indicates that in comparison with cells transfected with miR-NC, ^bP < 0.001; b indicates that in comparison with cells transfected with sh-FOXF2, ^bP < 0.001. FOXF2: Forkhead box F2; miR-NC: MiR negative control.