MiR-19a-3p regulates the Forkhead box F2-mediated Wnt/β-catenin signaling pathway and affects the biological functions of colorectal cancer cells

Figure 2 Effects of miR-19a-3p on the proliferation, invasion, migration, and epithelial-mesenchymal transition of colorectal cancer cells. A: miR-19a-3p was highly expressed in colorectal cancer cells; B: Compared with cells transfected with miR negative control (miR-NC), cells transfected with miR-19a-3p-mimics showed significantly upregulated expression of miR-19a-3p, and cells transfected with miR-19a-3p-inhibitor showed significantly downregulated expression of miR-19a-3p; C: Compared with cells transfected with miR-NC, cells transfected with miR-19a-3p-mimics showed significantly increased cell proliferation ability, and cells transfected with miR-19a-3p-inhibitor showed significantly decreased cell proliferation ability; D: Compared with cells transfected with miR-NC, cells transfected with miR-19a-3p-mimics showed significantly increased cell invasion ability, and cells transfected with miR-19a-3p-inhibitor showed significantly decreased cell invasion ability; E: Compared with cells transfected with miR-NC, cells transfected with miR-19a-3p-mimics showed significantly increased cell migration ability, and cells transfected with miR-19a-3p-inhibitor showed significantly decreased cell migration ability; F: Compared with cells transfected with miR-NC, cells transfected with miR-19a-3p-mimics showed significantly upregulated expression of p-GSK-3β and β-catenin, and significantly downregulated expression of GSK-3β and p-β-catenin, and cells transfected with miR-19a-3p-inhibitor showed significantly downregulated expression of p-GSK-3β and β-catenin, and significantly upregulated expression of GSK-3β and p-β-catenin; G: Western blot map; H: Compared with cells transfected with miR-NC, cells transfected with miR-19a-3p-mimics showed significantly upregulated expression of N-cadherin and vimentin, and significantly downregulated expression of E-cadherin, and cells transfected with miR-19a-3p-inhibitor showed significantly downregulated expression of N-cadherin and vimentin and significantly upregulated expression of E-cadherin; I: Western blot results. Note: a indicates that in comparison with cells transfected with miR-NC, ^bP < 0.001; b indicates that in comparison with cells transfected with miR-19a-3p-inhibitor, ^bP < 0.001. miR-NC: MiR negative control.