Reactive oxygen species-induced activation of Yes-associated protein-1 through the c-Myc pathway is a therapeutic target in hepatocellular carcinoma

Figure 4 The oncogenic action of yes-associated protein-1 was activated by the c-Myc pathway in reactive oxygen species-exposed hepatocellular carcinoma cells. A: Immunoblot analyses of phosphorylated-Akt, total-Akt, c-Myc, phosphorylated-p42/44 (Erk), total-p42/44 (Erk), and yes-associated protein-1 (YAP-1) were performed in reactive oxygen species (ROS)-exposed hepatocellular carcinoma (HCC) cells transfected with YAP-1 small interfering RNA (siRNA) or control siRNA. The experiment was repeated three times; B: Immunoblot analyses of phosphorylated-Akt, total-Akt, c-Myc, phosphorylated-p42/44 (p-Erk), total-p42/44 (Erk), and YAP-1 were performed in ROS-exposed HCC cells treated with verteporfin or control. The experiment was repeated three times. YAP: Yes-associated protein; siRNA: Small interfering RNA; VP: Verteporfin.