Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort

Figure 2 Deglycosylation assays of cyclooxygenase-2. A: Representative images of western blot of cyclooxygenase-2 (COX-2) protein levels corresponding to tumor tissue (T), negative control (NC) and deglycosylated tumor tissue (DT). B: Graphs correspond to upper or lower COX-2 bands quantifications. n = 4. C: Representative images of western blot of COX-2 protein levels in HT29 cells under basal conditions (C) or deglycosylated (DG). D: Graphs correspond to upper or lower COX-2 bands quantifications. n = 8. E. Relative levels of prostaglandin E₂ (PGE₂) normalized by total COX-2 band obtained in the corresponding western blot analysis. n = 11. Graphs show mean ± SD. ^aP ≤ 0.05, ^bP ≤ 0.01 and ^cP ≤ 0.001 vs the glycosylated condition. COX-2: Cyclooxygenase-2; T: Tumor; NC: Negative control; DT: Deglycosylated tumor; C: Cells under basal conditions; DG: Deglycosylated; PGE₂: Prostaglandin E₂.