Honokiol-enhanced cytotoxic T lymphocyte activity against cholangiocarcinoma cells mediated by dendritic cells pulsed with damage-associated molecular patterns

Figure 6 Dendritic cells pulsed with tumour cell lysates derived from honokiol-treated cholangiocarcinoma cells significantly enhanced T lymphocyte killing of cholangiocarcinoma cells. Differently primed effector T lymphocytes were cocultured with target KKU-213L5 cell line at ratios of 1:10 and 1:20. Killing capacity was evaluated using the MTT assay. A: Remaining target cells after coculture with effector cells for 48 h at 1:20 ratio photographed at 100×. Cytotoxic effect of T lymphocytes on KKU-213L5 is shown as % viability of target cells at (B) 24 h, (C) 48 h and for MMNK1 (D) at 48 h. Results are presented as mean ± SD of three independent experiments. A P value < 0.05 was considered significant; ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001. DCs: Dendritic cells; HOK: Honokiol; TCL: Tumour cell lysate.