Extract of Cycas revoluta Thunb. enhances the inhibitory effect of 5-fluorouracil on gastric cancer cells through the AKT-mTOR pathway

Figure 4 The mechanism of chemosensitization mediated by Cycas revoluta Thunb. extract. A: Hoechst 33258 staining to confirm apoptosis induced by 5-fluorouracil and the extract. The error bars represent ± STDEV from n = 3 independent experiments ^aP < 0.05, ^bP < 0.01; B: Western blot analysis of apoptosis-related proteins. Relative expressions are shown in the left histogram, and the error bars represent ± STDEV from n = 3 independent experiments ^aP < 0.05, ^bP < 0.01. 5-Fu: 5-fluorouracil; XIAP: X-linked inhibitor of apoptosis protein; AIF: Apoptosis inducing factor.