Relaxin influences ileal muscular activity through a dual signaling pathway in mice

Figure 1 Influence of oxadiazolo[4,3-a]-quinoxalin-1-one and 9-cyclopentyladenine mesylate on relaxin effects on the mechanical activity of the mouse ileum. A: Typical tracings showing the spontaneous activity of the ileal segments (Ctr, left hand recordings of each panel). RLX (50 nmol/L), 30 min from its application to the bath medium, causes a decrease in amplitude of the spontaneous contractions and a decay of the basal tension (a, right trace) compared to its control (a, left trace). Both these effects are reduced when RLX is added to the bath medium in the presence of 1 µmol/L ODQ (b, right trace) or 100 µmol/L 9CPA (c, right trace) and abolished in the presence of ODQ plus 9CPA (d, right trace). Data of each panel (a-d) are obtained from different preparations; B: Bar charts showing the influence of ODQ and 9CPA on RLX effects on the mean amplitude of spontaneous contractions (left hand histogram) and basal tension (right hand histogram) with respect to the control. All values are means ± SE of 6 preparations from 3 mice. ^aP < 0.05 vs Ctr; ^cP < 0.05 vs RLX (one-way ANOVA and Newman-Keuls post-test); RLX: Relaxin; ODQ: Oxadiazolo[4,3-a]-quinoxalin-1-one; 9CPA: 9-cyclopentyladenine.