Inhibitory effects of patchouli alcohol on stress-induced diarrhea-predominant irritable bowel syndrome

Figure 6 Effect of patchouli alcohol on a P2Y₁ receptor antagonist in EFS-induced contraction and ATP level of isolated colonic longitudinal smooth muscle. A-G: Mechanical recording, line chart, and histogram of the mechanism and effect of PA (100 μmol/L) on nerve-mediated rat colon contraction; H: Relaxation effect of α,β-MeATP and PA on EFS-induced contraction in IBS-D rats [control group (A): n = 32, IBS-D group (B): n = 31, α,β-MeATP-treated IBS-D group (C): n = 16, PA-treated IBS-D group (D): n = 8. ^aP < 0.05 vs control group, ^bP < 0.01 vs control group, ^dP < 0.01 vs group IBS-D; ANOVA]; I: Relaxation response of PA to MRS 2500-treated IBS-D colon [MRS 2500-treated IBS-D group (F): n = 13, PA + MRS 2500-treated IBS-D group (G), n = 15. ^aP < 0.05 vs MRS 2500-treated IBS-D group; unpaired t-test]; J: Effect of MRS 2500 on control and IBS-D rat colons [control group: n = 32, IBS-D group: n = 31, MRS 2500-treated control group (E): n = 13, MRS 2500-treated IBS-D group: n = 13. ^bP < 0.01 vs control group, ^dP < 0.01 vs group IBS-D; ANOVA]; K: ATP level of Kreb’s solution [control group: n = 23, IBS-D group: n = 17, α,β-MeATP-treated IBS-D group: n = 21, PA-treated IBS-D group: n = 8. ^aP < 0.05 vs control group, ^cP < 0.05 vs group IBS-D; ANOVA]; L: [Control group: n = 23, MRS 2500 treated control group: n = 12, IBS-D group: n = 17, MRS 2500-treated IBS-D group: n = 16. ^aP < 0.05 vs control group, ^cP < 0.05 vs group IBS-D; ANOVA], EFS: Electrical field stimulation; IBS-D: Irritable bowel syndrome; PA: Patchouli alcohol.