Involvement of methylation-associated silencing of formin 2 in colorectal carcinogenesis

Figure 1 Colorectal tissues show decreased formin 2 expression and a high frequency of hypermethylation in the formin 2 promoter. A: Normalized expression of formin 2 (FMN2) mRNA in carcinoma and adjacent normal tissues, presented as box-whisker plots (unpaired t-test, GEO: GSE20842); B: Normalized expression of FMN2 mRNA in tissue from different stages of carcinoma (according to the AJCC Cancer Staging Manual) and in normal tissue, presented as box-whisker plots (unpaired t-test, colorectal cancer samples from TCGA); C: Normalized expression of FMN2 mRNA in adenoma and corresponding normal colonic mucosal tissues, presented as box-whisker plots (unpaired t-test, GEO: GSE8671); D: Normalized expression of FMN2 mRNA in normal, adenoma, and carcinoma tissues, presented as box-whisker plots (unpaired t-test, GEO: GSE4183); E: The FMN2 gene shows an increased methylation level in colorectal cancer tissues compared with normal tissues (unpaired t-test, colorectal cancer samples from TCGA); F: Receiver operating characteristic curve analysis was used to assess the clinical diagnostic utility of FMN2 DNA methylation for the prediction of colorectal cancer.