Ubiquitin-like modifier activating enzyme 2 promotes cell migration and invasion through Wnt/β-catenin signaling in gastric cancer

Figure 7 Alterations in Wnt/β-catenin signaling and related protein expression levels in BGC-823 and SGC-7901 cells. A: Western blotting showed that expression of proteins related to the Wnt/β-catenin signaling pathway was altered in BGC-823 cells transfected with siRNA and SGC-7901 cells with Uba2 over-expression; B: Luciferase reporter assays of BGC-823 cells transfected with siRNA and SGC-7901 cells with Uba2 over-expression after transfection with TOP Flash or FOP Flash and Renilla pRL-TK plasmids. Reporter activity was normalized to Renilla luciferase activity. ^aP < 0.01 vs Control; ^bP < 0.01 vs EV. EV: Empty vector; Uba2: Ubiquitin-like modifier activating enzyme 2.