Daikenchuto (Da-Jian-Zhong-Tang) ameliorates intestinal fibrosis by activating myofibroblast transient receptor potential ankyrin 1 channel

Figure 7 Extracellular collagen upregulates transient receptor potential ankyrin 1 expression to exert anti-fibrotic effects. A: Type I collagen solution (0, 0.1, or 1 μg/mL) was added into the culture medium, and immunoblotting was performed to detect TRPA1 and Type I collagen expression in InMyoFibs. The rightmost graph shows the summary (n = 4) B-E: Relative expression levels (shown as the post/pre ratio) of col1a1, trpa1, myocd, and acta2 mRNAs quantified by RT-PCR in Type Icollagen- or TGF-β1-treated InMyoFibs. In E, InMyoFibs were pre-treated with TRPA1 siRNA. ^aP < 0.05 vs control cells; ^bP < 0.05 vs TGF-β1-treated cells (n = 4). TRPA1: Transient receptor potential ankyrin 1; InMyoFibs: Intestinal myofibroblasts; TGF-β1: Transforming growth factor-β1.