Total polysaccharides of the Sijunzi decoction attenuate tumor necrosis factor-α-induced damage to the barrier function of a Caco-2 cell monolayer via the nuclear factor-κB-myosin light chain kinase-myosin light chain pathway

Figure 4 Total polysaccharides of the Sijunzi decoction regulated the secretion of inflammatory cytokines by Caco-2 cells. Caco-2 cells were incubated with or without 100 ng/mL TNF-α in the absence or presence of TPSJ for 24 h. Cell culture media were collected, and the concentrations of TNF-α (A), IL-6 (B), and IL-8 (C) were detected by ELISA (^bP < 0.01, ^eP < 0.001 vs control Caco-2 cells; ^cP < 0.05, ^fP < 0.001 vs TNF-α-damaged Caco-2 cells). TPSJ: Total polysaccharides of the Sijunzi decoction; TNF: Tumor necrosis factor.