Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway

Figure 4 Effect of caffeic acid phenethyl ester on Nrf2 expression in hepatic stellate cell-T6 cells. A: HSC-T6 cells were treated with 5 μmol/L, 10 μmol/L and 15 μmol/L CAPE for 24 h. Nrf2 mRNA and cytosol and nuclear protein expression levels were investigated using real-time PCR and Western blot, respectively; B: The nucleus/cytosol ratio defines Nrf2 protein expression in the nucleus/Nrf2 protein expression in cytosol; C: Indirect immunofluorescence (× 200) analysis of Nrf2 protein expression in HSC-T6 cells. The upper: control group and the lower: 15 μmol/L CAPE group. The data presented are the mean ± SD (n = 3). ^aP < 0.05 vs control. CAPE: Caffeic acid phenethyl ester.