Effect of toll-like receptor 3 agonist poly I:C on intestinal mucosa and epithelial barrier function in mouse models of acute colitis

Figure 4 Effect of poly I:C on colonic epithelial junctions. A: Representative photographs of immunohistochemistry staining for zo-1, occludin and claudin-1 (Magnification: × 200); B: Representative photographs of immunofluorescent staining for zo-1 (green), occludin and claudin-1 (red); C: Expressions of zo-1, occludin and clandin-1 at the mRNA level, as analyzed by RT-qPCR; D: Protein expressions of zo-1, occludin and clandin-1 as analyzed by Western Blot. Data are expressed as mean ± SD. ^bP < 0.01 vs control group; ^dP < 0.01 vs model group. RT-qPCR: Real-time quantitative polymerase chain reaction.