Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway

Figure 4 Inhibition of miR-30a-3p/5p expression promotes esophageal squamous cell carcinoma cell proliferation. A: miR-30a-3p and miR-30a-5p were knocked down using miR-30a inhibitor in KYSE150 cells (top), and inhibition of miR-30a expression promoted KYSE150 cell proliferation, as indicated by the MTT assay (bottom); B: Inhibition of miR-30a expression promoted KYSE150 cells proliferation, as indicated by the colony formation assay. Colonies containing more than 50 cells were counted; C: Inhibition of miR-30a expression promoted anchorage-independent growth ability of KYSE150 cells, as indicated by the soft-agar assay. Colonies containing more than 50 cells were counted; D: Subcutaneous tumorigenesis assay performed in nude mice (n = 6/group) indicated that inhibition of miR-30a expression promoted ESCC growth in vivo. Tumor volumes were measured on the 1^st, 5^th, 10^th, 15^th, and 20^th d; E and F: Statistical analyses of tumor volumes in the negative control and miR-30a over-expression groups. Error bars represent the mean ± SD of three independent experiments; ^aP < 0.05.