Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway

Figure 3 Over-expression of miR-30a-3p/5p represses esophageal squamous cell carcinoma cell proliferation. A: miR-30a expression in human esophageal epithelial cell line Het-1A and ESCC cell lines KYSE30 and KYSE150; B: miR-30a-3p (left) and miR-30a-5p (right) were, respectively, over-expressed in KYSE30 cells; C: Over-expression of miR-30a suppressed KYSE30 cells proliferation, as indicated by the MTT assay; D: Over-expression of miR-30a suppressed KYSE30 cell proliferation, as indicated by the colony formation assay. Colonies containing more than 50 cells were counted. E: Over-expression of miR-30a suppressed anchorage-independent growth ability of KYSE30 cells, as indicated by the soft-agar assay. Colonies containing more than 50 cells were counted. F: Subcutaneous tumorigenesis assay performed in nude mice (n = 6/group) indicated that over-expression of miR-30a inhibited ESCC growth in vivo. Tumor volumes were measured on the 1^st, 5^th, 10^th, 15^th, and 20^th d; G and H: Statistical analyses of tumor volumes in the negative control and miR-30a over-expression groups. Error bars represent the mean ± SD of three independent experiments; ^aP < 0.05.