Mitofusin-2 mediated mitochondrial Ca2+ uptake 1/2 induced liver injury in rat remote ischemic perconditioning liver transplantation and alpha mouse liver-12 hypoxia cell line models

Figure 4 Mfn2 knockdown alters MICUs expression in AML-12 hypoxia cell lines. A: Mfn2 knockdown was tested by WB and qRT-PCR. Expression of MICUs in AML-12 hypoxia cell lines was measured by WB (B) and qRT-PCR (C). Taking the hypoxia group as the reference, both the NC and hypoxia + Si groups showed higher expression of MICU-1 and similar expression of MICU-2; MCU altered expression in WB, but no significant change in qRT-PCR. AML: Alpha mouse liver; MCU: Mitochondrial Ca²⁺ uniporter; Mfn2: Mitofusin-2; MICU: Mitochondrial Ca²⁺ uptake; NC: Normal culture; qRT-PCR: Quantitative real-time-PCR; WB: Western blotting. ^aP < 0.05; ^bP < 0.01.S ^cP < 0.001.