(-)-Epigallocatechin-3-gallate enhances poly I:C-induced interferon-λ1 production and inhibits hepatitis C virus replication in hepatocytes

Figure 5 Effect of (-)-epigallocatechin-3-gallate on poly I:C-induced ISGs expression in JFH-1-infected Huh7 cells. JFH-1-infected Huh7 cells (72 h post-infection) were treated with EGCG at the indicated concentrations for 1 h prior to poly I:C (1 μg/mL) stimulation. Total RNA extracted from the cells after 24 h of stimulation was subjected to real-time reverse transcription-polymerase chain reaction for the determination of ISG and glyceraldehyde-3-phosphate dehydrogenase mRNA levels. The data are expressed as ISG15 (A), ISG56 (B), MxA (C), and OAS-1 (D) mRNA levels relative (fold) to the control (vehicle only, which is defined as 1). The results shown are the mean ± SD of triplicate measurements representative of three experiments (Poly I:C vs LyoVec, ^aP < 0.05, ^bP < 0.01). EGCG: (-)-Epigallocatechin-3-gallate.