Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model

Figure 5 Effect of the single amino acid mutant on high-density lipoprotein absorption by class B scavenger receptor I. Huh7-siSR-BI cells were seeded in a 96-well plate and cultured overnight prior to transfection. Cells were transfected with the SR-BI wild type (WT) and mutant S112F plasmids using LipofectamineTM. Complete culture medium was replaced 6 h after transfection and cells were cultured for 48 h before DiI-HDL (200 μg/mL) was added, and the cells were cultured for an additional 4 h. Nuclei were stained with Hoechst for 10 min, and cells were observed and images captured using a microscope (^bP < 0.01). SR-BI: Class B scavenger receptor I.