miR-29a promotes hepatitis B virus replication and expression by targeting SMARCE1 in hepatoma carcinoma

Figure 4 Effect of SMARCE1 on hepatitis B virus replication and expression (n = 3). A: HepG2.2.15 cells were transfected with pcDNA-control, pcDNA-SMARCE1, si-control, or si-SMARCE1 for 24 h; B and E: Western blotting was performed to detect the level of SMARCE1 after pcDNA-SMARCE1 or si-SMARCE1 transfection; C and D: qPCR and Southern blotting analysis showed that SMARCE1 overexpression significantly reduced the HBV DNA replication and SMARCE1 knockdown significantly increased the HBV DNA replication; F and G: ELISA assay showed that SMARCE1 overexpression significantly decreased the levels of HBsAg and HBeAg; H and I: Endogenous SMARCE1 inhibition by si-SMARCE1 significantly promoted the levels of HBsAg and HBeAg. ^aP < 0.05 vs controls. Data represent the mean ± SD.