Copyright
©The Author(s) 2017.
World J Gastroenterol. Jun 21, 2017; 23(23): 4243-4251
Published online Jun 21, 2017. doi: 10.3748/wjg.v23.i23.4243
Published online Jun 21, 2017. doi: 10.3748/wjg.v23.i23.4243
Figure 5 Overexpression miR-382 inhibited migration, invasion and epithelial-mesenchymal transition in Eca109 cells.
A: Representative photomicrographs showed migrated cells stained with crystal violet after post-culture for 24 h in the transwell migration assay. The bars represent 200 μm; B: The migrated cells in five different fields were counted and the Y-axis represents the migrated cell number from three independent experiments. Data is presented as mean ± SD, bP < 0.01; C: Representative photomicrographs showing invaded cells stained with crystal violet after post-culture for 24 h in the transwell invasion assay. The bars are 200 μm; D: The invaded cells in five different fields were counted and the Y-axis represents the invaded cell number from three independent experiments. Data are presented as mean ± SD, aP < 0.05; E: Western blot analysis of the expression of epithelial marker E-cadherin, and mesenchymal markers β-catenin, vimentin and snail in Eca109 cells infected with LV-miR-382 or LV-Con(c). β-actin served as a loading control.
- Citation: Feng J, Qi B, Guo L, Chen LY, Wei XF, Liu YZ, Zhao BS. miR-382 functions as a tumor suppressor against esophageal squamous cell carcinoma. World J Gastroenterol 2017; 23(23): 4243-4251
- URL: https://www.wjgnet.com/1007-9327/full/v23/i23/4243.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i23.4243