Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice

Figure 6 Upregulation of IL-13Rα2 expression in 5-FU treated Fn14 KO mice. A: Fold increase of IL-13Rα2 and IL-13Rα1 mRNA in total ileum from WT or Fn14 KO mice after 5-FU treatment (n = about 3-5 mice per group). Data are shown as mean ± SD; B: Representative images from immunohistochemistry of IL-13Rα2 in ileum from WT and Fn14 KO mice on days 0 (naïve), 5, and 8 after injection of 5-FU. Green, IL-13Rα2; red, nuclei. Scale bar = 100 µm; C: Quantification of IL-13Rα2 expression. The IL-13Rα2-positive area of images from the experiment described in (B) were quantified following normalization by mucosal length (n = 4 mice per group). ^aP < 0.05; D: Recombinant IL-13 (400 µg) was injected into naïve WT or Fn14 KO mice. After 3 d, ileum samples were collected, and sections were probed with anti-IL-13Rα2 antibody. Green, IL-13Rα2; red, nuclei. Scale bar = 100 µm; E: Quantification of IL-13Rα2 expression normalized to mucosal length. The IL-13Rα2-positive area of images from the experiment described in (D) were quantified (n = 4 mice per group). There was no statistically significant difference between WT and Fn14 KO mice.