miR-34a mediates oxaliplatin resistance of colorectal cancer cells by inhibiting macroautophagy via transforming growth factor-β/Smad4 pathway

Figure 6 Activation of autophagy protected colorectal cancer cells against oxaliplatin-induced apoptosis by inhibiting miR-34a through the TGF-β/Smad4 pathway. A: HT29 and HT29-oxaliplatin (OXA) cells were transfected with miR-34a mimics and treated with OXA (10 μmol/L) and simultaneously mixed with or without 3-MA. After 48 h, the apoptosis rate was examined by flow cytometry; B: The above cell lysates were examined with the indicated antibodies by western blotting. β-actin was used as loading control. ^eP < 0.001 indicates a significant difference vs normal control; ^cP < 0.05 indicates a significant difference vs group treated with OXA.