miR-34a mediates oxaliplatin resistance of colorectal cancer cells by inhibiting macroautophagy via transforming growth factor-β/Smad4 pathway

Figure 5 miR-34a inhibited oxaliplatin-induced autophagy in colorectal cancer cells. A: HT29 and HT29-oxaliplatin (OXA) cells were transfected with miR-34a mimics and treated with or without OXA (10 μmol/L) simultaneously. After 48 h, autophagy was determined using a fluorescent dye (monodansylcadaverine, MDC) and flow cytometry; B: The above cell lysates were examined with indicated antibodies by western blotting. β-actin was used as loading control. (^eP < 0.001 indicates a significant difference vs normal control; ^cP < 0.05 indicates a significant difference vs transfection with miR-34a mimic group).