Review
Copyright ©The Author(s) 2016.
World J Gastroenterol. Feb 21, 2016; 22(7): 2284-2293
Published online Feb 21, 2016. doi: 10.3748/wjg.v22.i7.2284
Table 1 Commercial next-generation sequencing platforms for human genome sequencing
454 GS FLXGAIIxMiSeqHiSeq 2500SOLiD 5500Ion PGMIon ProtonPacBio RS
(318 chip)
Reads per run1 M150 M50 M6 G1.4 G5.5 M60 M50 K
Read length (bp)7002 × 1502 × 1502 × 1002 × 50400200250-10000
Output per run700 Mb90 Gb15 Gb600 Gb120 Gb2 Gb12 Gb200 Mb
Run time24 h14 d55 h10 d7 d5 h3 h2 h
Cost/Mb1$10.00$0.15$0.50$0.05$0.10$1.00$0.08$2.00
AdvantageLong read lengthWidly usedWidly usedHigh-throughput, widly usedHigh-throughput, accuracyFast, flexible chipHigh-throughput, fastLong read length, fast
DisadvantageLong hand-on time, low outputLong run timeLong run timeLong run timeLong run time, short read lengthHigh error rate (homopolymer)High error rate (homopolymer)High error rate