Case Control Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Jul 21, 2016; 22(27): 6246-6256
Published online Jul 21, 2016. doi: 10.3748/wjg.v22.i27.6246
Table 1 Antibodies and immunohistochemistry parameters used in this study
AntibodyManufacturerCloneSpeciesDilutionRetrieval1Positive controlStaining pattern
EGFR PharmDxDako2-18C9MousePre-dilutedProteinase KProvided by manufacturerMembrane
Ki67 AgDakoMIB-1Mouse1:400StandardTonsilNucleus
Caspase 3DBS3C SP03Mouse1:400StandardTonsil/stomachCytoplasm
ERK1DBSPolyclonalRabbit1:100StandardBreast carcinomaCytoplasm
ERK2DBSPolyclonalRabbit1:400StandardBreast carcinomaCytoplasm
METCell signalingPolyclonalRabbit1:50StandardBreast carcinomaCytoplasm
mTORCell signalingPolyclonalRabbit1:50StandardLymphoid tissueCytoplasm
K19Novocastrab170Mouse1:300StandardCholangio-carcinomaCytoplasm
VimentinDakoVim3B4Mouse1:3000StandardKidneyCytoplasm
p53DakoDO7Mouse1:100StandardBreast carcinomaNucleus
Beta-cateninBD14Beta-cateninMouse1:800StandardTonsilMembrane/nucleus
Cyclin D1DakoSP4Rabbit1:100Steamer 10 mmol/L TRIS, 1 mmol/L EDTA, pH 9Tonsil/intestineNucleus