Avoiding hepatic metastasis naturally: Lessons from the cotton top tamarin (Saguinus oedipus)

Figure 4 Specific carcinoembryonic antigen bands shared by cotton top tamarin and humans. Western blot using solid phase anti-CEA monoclonal antibody. lmmunoblotting was performed using 5.6 pg protein/ml antibody after electrophoresis of a 12% SDS-polyacrylamide gel run under reducing conditions. Relative mobility (M) is shown on the left, and the type of samples loaded at 10 pg protein/lane are shown above the numbered lanes. Cotton-top tamarin extracts are in lanes 1 to 4 and effluent samples in 7 and 8. An extract from a patient with histologically proven rectal cancer and IBD is in lane 9, with human effluent samples in lanes I0 and 11 and human meconium in lane 12. Lane 5 contains the positive human CEA control, and the M, markers (M) are in lane 6. An arrow indicates the M, - 50000 band evident also in human extract (lane 5) likely a deglycosylated moiety. (Published with permission of Elsevier[67] and modified). CEA: Carcinoembryonic antigen.