MicroRNA-548a-5p promotes proliferation and inhibits apoptosis in hepatocellular carcinoma cells by targeting Tg737

Figure 2 Tg737 is a target gene of miR-548a-5p. A: MiR-548a-5p and its deductive binding domain in the 3'UTR of Tg737; B: Relative expression of miR-548a-5p; C: qRT-PCR and Western blot analysis demonstrated that miR-548a-5p overexpression significantly decreased Tg737 mRNA expression and miR-548a-5p knockdown significantly increased Tg737 mRNA expression in HepG2 and MHCC97H cells; D: Luciferase reporter assay of the connection between the 3'UTR of Tg737 and miR-548a-5p in HepG2 and MHCC97H cells; E: Western blot analysis demonstrated higher miR-548a-5p and lower Tg737 expression in human HCC specimens compared to normal liver tissue; F: qRT-PCR demonstrated higher miR-548a-5p and lower Tg737 expression in human HCC specimens compared to normal liver tissue; G: Spearman’s correlation assay showed that miR-548a-5p was negatively correlated with Tg737 mRNA expression in HCC specimens. ^aP < 0.05, ^bP < 0.01 vs control.