Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review

Table 3 Global status of multiplex qPCR developed for hepatitis viral infections with and without other pathogens

No.	Assay systems	Instruments used	Group of pathogens detected		Types of chemistries/detection methods used	Ref.
			Hepatitis viruses	Other pathogens
1	Multiplex real time PCR	Mx4000 (Stratagene)	HBV, HCV	HIV type-1, T. pallidum	TaqMan-LNA probe	[21]
2	Multiplex real time PCR	Light cycler 480 (Roche)	HEV genotypes	-	N.A.	[41]
3	Real time PCR assay	ABI 7500 (Applied Biosystems)	HAV, HBV, HCV, HDV, HEV	-	TaqMan Array card	[42]
4	Multiplex qPCR assay	Light cycler 480 (Roche)	HBV, HDV	-	TaqMan probe	[43]
5	Multiplex qPCR assay	ABI 7500 (Applied Biosystems)	HAV, HEV	-	Hydrolysis probe	[22]
6	Multiplex qRT-PCR	N.A.	HAV	Norovirus genotypes 1 and 2	TaqMan probe	[44]
7	Multiplex ligation dependent probe real time PCR	Rotor-GeneQ (Qiagen)	HBV mutants	-	TaqMan probe	[45]
					MLPA probe
8	Multiplex real time RT-PCR	N.A.	HEV genotypes	-	N.A.	[46]
9	Multiplex qPCR	N.A.	HBV genotypes	-	SYBR Green	[47]
10	Multiplex Real time PCR	N.A.	HAV	Norovirus, Rotavirus, Coxsackievirus	TaqMan probe	[48]
11	Multiplex Real time PCR	Light cycler 2.0 (Roche)	HAV, HBV, HCV and HEV	-	FRET probe	[15]
12	Multiplex RT-PCR	ABI 2720 (Applied Biosystems)	HCV	HIV type-1	SYBR Green I	[8]
13	Multiplex qPCR	N.A.	HAV, HEV	Entero and Adeno-viruses	N.A.	[49]
14	Multiplex Real-Time PCR Assay	CFX96 (Bio-Rad)	HAV, HBV, HCV	-	READ technology based fluorophore	[4]
15	RT PCR assay	Smart cycler II (Cepheid)	HBV, HCV	-	TaqMan probe	[50]
16	Duplex real time PCR	ABI 7500 (Applied Biosystems)	HBV variants	-	Hydrolysis probe	[51]
17	Multiplex RT PCR	N.A.	HCV subtyping	-	Electrophoresis	[52]
18	Multiplex qPCR	N.A.	HBV genotypes	-	N.A.	[53]
19	Multiplex qPCR	N.A.	HCV	HIV type-1	SYBR Green I	[54]
20	Duplex real-time RT-PCR	ABI Prism system (Applied Biosystems)	HCV variants	-	Hydrolysis probe	[55]
21	Multiplex real time PCR	N.A.	HAV	Norovirus genotypes 1 and 2	N.A.	[56]
22	Duplex real-time qRT-PCR	ABI Prism 7000 (Applied Biosystems)	HAV	MS2 bacteriophage	MGB-TaqMan probe	[57]
23	Multiplex TaqMan RT-qPCR system	MX30005P (Stratagene)	HEV	FCV	TaqMan probe	[58]
24	Multiplex real time PCR	ABI 7300 (Applied Biosystems)	HBV genotypes	-	TaqMan probe	[59]
25	Real time PCR	N.A.	HBV genotypes	-	TaqMan probe	[60]
26	Multiplex real time PCR	Mx3005P (Stratagene)	HEV	FCV	TaqMan probe	[61]
27	Multiplex RT PCR assay	ABI Prism 7500 (Applied Biosystems)	HCV	PDV	MGB hybridization probe	[62]
28	Multiplex qPCR assay	N.A.	HBV	B19, HHV-8, EBV, CMV, VZV	N.A.	[63]
29	Multiplex qPCR	N.A.	HBV, HCV	HIV type-1	SYBR Green I	[16]
30	Multiplex Real Time PCR	ABI 7500 (Applied Biosystems)	HBV mutants	-	LNA probes with SYBR Green I	[64]
31	Microarray multiplex assay	ABI Prism 7700 (Applied Biosystems)	HBV, HCV	HIV type-1	Oligonucleotide array labeled with Cy5 and Cy3	[65]
32	Real time multiplex PCR	N.A.	HAV	Entero and Adeno-viruses	Probes labeled with FAM, R6G, ROX, Cy5	[66]
33	Multiplex real time RT-PCR	LightCycler (Roche)	HCV	HIV type-1	SYBR Green	[67]
34	Real time multiplex PCR	icycler iQ (Bio-Rad)	HCV variants	-	TaqMan probes	[68]
35	Multiplex real-time RT PCR	ABI 7000 (Applied Biosystems)	HCV genotypes	-	Primer probes	[69]
36	Multiplex real-time qPCR	Mx4000 (Stratagene)	HBV, HCV	HIV type-1	TaqMan probes	[70]
37	Automated multiplex PCR	ABI Prism 7700 (Applied Biosystems)	HBV, HCV	HIV type-1	TaqMan probes	[71]