Review
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 28, 2016; 22(20): 4824-4834
Published online May 28, 2016. doi: 10.3748/wjg.v22.i20.4824
Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review
Mohammad Irshad, Priyanka Gupta, Dhananjay Singh Mankotia, Mohammad Ahmad Ansari
Mohammad Irshad, Priyanka Gupta, Dhananjay Singh Mankotia, Mohammad Ahmad Ansari, Clinical Biochemistry Division, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi 110029, India
Author contributions: Gupta P collected the information from published literature; Mankotia DS and Ansari MA categorized it under different sub-headings and prepared the manuscript; Irshad M edited the manuscript and corrected/modified the language.
Conflict-of-interest statement: There is no conflict of interest among the authors of this study.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Mohammad Irshad, Professor, Clinical Biochemistry Division, Department of Laboratory Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. drirshad54@yahoo.com
Telephone: +91-11-26594981 Fax: +91-11-26588663
Received: February 9, 2016
Peer-review started: February 9, 2016
First decision: March 7, 2016
Revised: March 9, 2016
Accepted: March 30, 2016
Article in press: March 30, 2016
Published online: May 28, 2016
Core Tip

Core tip: The present review describes the worldwide application and the significance of multiplex quantitative real time polymerase chain reaction (qPCR) for simultaneous detection of hepatitis viruses and their subtypes in serum. The published literature has demonstrated that the multiplex qPCR assay is a fast, easy, cost-effective, and sensitive technique for the early diagnosis of hepatitis co-infections. Use of this technique, in comparison to other diagnostic procedures, is increasing in diagnostic laboratories.