Growth hormone abolishes the negative effects of everolimus on intestinal wound healing

Figure 3 Azan staining from the anastomotic region. The collagen fibres appear blue. Everolimus treated animals show a disturbed arrangement of collagen fibres, which is antagonized by additional human growth hormone (hGH)-treatment (A-C). A: Vehicle; B: Everolimus 3.0 mg/kg; C: Everolimus 3.0 mg/kg + hGH 2.5 mg/kg. Hydroxyproline concentration in the anastomotic region in mg per mg dry weight (DW). The decrease of hydroxyproline under everolimus-treatment is reduced by additional hGH-treatment (D) (magnification × 400). V: Vehicle, E: Everolimus 3.0 mg/kg, E + GH: Everolimus 3.0 mg/kg + hGH 2.5 mg/kg; ^aP < 0.05, vs E group, V vs E + GH group.