Minireviews
Copyright ©The Author(s) 2016.
World J Gastroenterol. Jan 7, 2016; 22(1): 417-426
Published online Jan 7, 2016. doi: 10.3748/wjg.v22.i1.417
Table 1 Advantages and disadvantages of nuclear magnetic resonance and mass spectroscopy
NMRMS
Sensitivity (detection limit)Usually micromolar (nanomolar with cryosonde)Picomolar
ReproducibilityHighLow
DetectedNon targeted approachTargeted approach
MetaboliteDetect metabolite Only if contain proton on the moleculeNeed specific preparation to well detected some metabolites (Lipids…)
Metabolite identificationEasy, using 1D and/or 2D spectra and databasesMore difficult, need sometime complementary analysis
Number of know identifiable metabolitesMore than 200More than 4000
SampleSimple preparation (minimal add of D2O, Buffer and sometime reference)Preparation more complex (protein extraction, etc.)
Non destructive methodDestructive method
Need 400 μL (less than 10 μL with microprobe)Need few microliters
Type of sampleLiquid (urine, whole blood, serum, plasma, etc.) and intact tissueLiquid
Cost of machineVery highHigh
Cost of sample analysisLowerHigher
Signal acquisition time5 to 15 min for 1D spectraAround 10 min
More longer for 2D spectra (few hours)