S-phase arrest after vincristine treatment may promote hepatitis B virus replication

Figure 8 Vincristine at 0. 1 μmol/L does not cause significant cell apoptosis. HepG2.2.15 (4 × 10⁵/well) and HepG2-HBV1.1 (6 × 10⁵/well) cells were treated with vincristine at a concentration of 0.1 μmol/L for 24 h, followed by 48 h of incubation in drug-free culture medium. A: The level of apoptosis in the HepG2.2.15 cells was detected based on flow cytometry; B: The level of apoptosis in the HepG2-HBV1.1 cells was detected based on flow cytometry; C: Western blot analysis of the PARP protein. PARP: Poly(ADP-ribose) polymerase; LR: Lower-right; UR: Upper-right.