S-phase arrest after vincristine treatment may promote hepatitis B virus replication

Figure 4 Vincristine promotes HepG2-HBV1. 1 cell excretion of hepatitis B virus nucleocapsids instead of hepatitis B virus Dane particles. HepG2-HBV1.1 cells (6 × 10⁵/well) were treated with vincristine at a concentration of 0.1 μmol/L for 24 h followed by 48 h of incubation in drug-free culture medium. The HBcAg from HBV Dane particles or nucleocapsids in the supernatant was analyzed using an ELISA assay. Dane: HBcAg from HBV Dane particles; Dane + nucleocapsid: HBcAg from HBV Dane particles and nucleocapsids. Values represent the mean ± SD of 3 independent experiments. ^aP < 0.05, ^bP < 0.01 vs control group (no vincristine). HBeAg: Hepatitis B e Antigen.