Effects of Ligustrum robustum on gut microbes and obesity in rats

Table 1 Culture media and incubation conditions

Bacterial group	Bacteria preparation		MIC
	Medium	Incubation conditions	Medium	Incubation conditions
Bacteroides	GAM1 agar	37 °C anaerobic, 48 h	BHI2 (10% HS4)	37 °C Anaerobic, 48 h
Bifidobacteria	BBL2 agar	37 °C anaerobic, 48 h	RC2	37 °C Anaerobic, 48 h
Lactobacilli	LBS2 agar	37 °C aerobic with 5% CO2 48 h	RC2	37 °C Anaerobic, 48 h
C. perfringens	SPS2 agar	37 °C anaerobic, 48 h	RC2	37 °C Anaerobic, 48 h
Aerobic E. coli	EMB2 agar	37 °C aerobic, 18 h	MH2	37 °C Aerobic, 18 h
Enterococci	BEA2 agar	37 °C aerobic, 18 h	MH2	37 °C Aerobic, 18 h
Candida albicans	Salouraud2 agar	37 °C aerobic, 18 h	Salouraud2	37 °C Aerobic, 18 h
Staphylococcus aureus	blood plate3	37 °C aerobic, 18 h	MH2	37 °C Aerobic, 18 h
Other bacteria	LB1 agar	37 °C aerobic, 18 h	MH2	37 °C Aerobic, 18 h

¹Prepared in the laboratory according to methods defined by the Chinese Ministry of Health[8];

²Purchased from Beijing Land Bridge Technology Co., Ltd., China;

³Purchased from Chengdu RICH Technology Co., Ltd., China;

⁴Purchased from Hyclone. MIC: Minimum inhibitory concentration; GAM: Gifu anaerobic medium; BBL: Bifidobacterium culture; LBS: Lactobacillus selection; SPS: Sulfite polymyxin-sulfadiazine; EMB: Eosin methylene blue; BEA: Bile esculin azide; LB: Luria-Bertani; BHI (10% HS): Brain heart infusion with 10% horse serum; RC: Reinforced Clostridium medium; MH: Mueller-Hinton medium.