Heat shock pretreatment improves stem cell repair following ischemia-reperfusion injury via autophagy

Figure 6 Heat shock pretreatment increases the homing and survival rate of transplanted mesenchymal stem cells in I/R livers in vivo. CM-Dil-labeled positive cells (red color, B1, C1), PCNA-conjugated with FITC (green color, B2, C2) and their co-localization (yellow color, B3, C3) were detected by immunofluorescence microscopy, respectively (magnification × 100). The total number of double-positive cells labeled by CM-Dil and PCNA in the heat shock pretreatment-mesenchymal stem cell (MSC)-treated group was higher than that in the MSC-treated group. The arrows indicate positive stained cells by CM-Dil, PCNA or their co-localization, respectively. The data represent the results of three separate experiments.