Calcium glycerophosphate preserves transepithelial integrity in the Caco-2 model of intestinal transport

Table 1 Baseline changes in trans epithelial electrical resistance and mannitol flux from time 0 to 4 h

Treatment	Rate of TEER change	Rate of mannitol flux
	% per hour	dpm per hour
Control	0.69 ± 0.11a	28 ± 4
Vehicle	-0.24 ± 0.32c	19 ± 4
1 mmol/L CGP	0.80 ± 0.14a	31 ± 4
100 μmol/L CGP	0.28 ± 0.13	19 ± 3
10 μmol/L CGP	-.09 ± 0.37	21 ± 5
1 μmol/L CGP	0.48 ± 0.23c	31 ± 6

Trans epithelial electrical resistance (TEER) was significantly lower in vehicle (0.1% citric acid) treated cells, an effect that was reversed by calcium glycerophosphate (CGP) in a concentration dependent fashion.

^aP < 0.05 vs vehicle;

^cP < 0.05 vs control. Rate of TEER and mannitol flux were calculated by taking the slope of the linear portion of the TEER or mannitol flux vs time curve (0 to 4 h). dpm represents disintegrations per unit time. Each value represents the results of three independent experiments. Data is expressed as mean ± SE.