Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro

Figure 3 Expression of HMGB1 protein pA-box mRNA in transfected SW480 cells. After SW480 cells were transfected for 48 h, HMGB1 pA-box mRNA levels were determined by real-time PCR. The expression of HMGB1 A-box mRNA in the pA-box-transfection SW480 cells was significantly upregulated compared with the control and mock-transfection groups. The data are expressed as the mean ± SD and are derived from three independent experiments, which were each performed in duplicate. The means with asterisks above them are significantly different (^bP < 0.01 vs control).