Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Figure 6 Butein inhibited the interleukin-6-induced activation of signal transducer and activator of transcription 3 in Colo 205 cells. A: Colo 205 cells were treated with various concentrations of interleukin (IL)-6; B: Colo 205 cells were treated with IL-6 for various times; C: Expression levels of phosphorylated signal transducer and activator of transcription 3 (pSTAT3), were measured by Western blot analysis after pretreating Colo 205 cells with IL-6 (25 ng/mL) for 1 h prior to butein treatment for 24 h at various concentrations (n = 3).