Alterations in serotonin, transient receptor potential channels and protease-activated receptors in rats with irritable bowel syndrome attenuated by Shugan decoction

Figure 6 Endocrine cells in colonic tissues. A, C: 5-HT levels in colonic tissues measured by IHC; B, D: CgA expression in colon tissues measured by immunohistochemistry. Data are presented as mean ± SD, ^aP < 0.05 vs control; ^dP < 0.01 vs IBS, Statistical comparisons were performed using One-way ANOVA followed by Dunnett’s test using GraphPad Prism 5.0.; ^fP < 0.01 vs TXYF, difference between SGD-M and TXYF was analyzed by Student’s t test using GraphPad Prism 5.0. (n = 3-5). 5-HT: 5-hydroxytryptamide; IHC: Immunohistochemistry; IBS: Irritable bowel syndrome; SGD: Shugan decoction; TXYF: TongXieYaoFang.